The Jamaican star kicked off his Gold Coast 2018 campaign by qualifying fastest for the final after winning his semifinal heat in 10.06s yesterday.
He was a class apart as he galloped over the finish line in 10.03.
Blake rounded off the podium in third place with a time of 10.19. 'All over the place' "Maybe I was too confident and over thought it, " said Blake, who appeared to be uncharacteristically quiet behind the blocks.
"I've been feeling good and it was not to happen today". The path to gold was made smoother for Simbine when England runner Adam Gemili, a 4x100m relay gold medalist at the London world championships, pulled out before the final with a thigh injury.
Simbine who was 5th at the 2016 Olympics won in 10.03 secs while his compatriot Bruintjies returned 10.17 sec.
Blake was forced into damage control as he clawed back the competition to cross the line for third behind South Africa's Akani Simbine (gold) and Henricho Bruinjies (silver).
This race had been billed as a chance for Blake to win his first major individual title since the 2011 World Championships and Bolt had warned his former training partner: "You've got to win or you can't go home".
"Yohan is a great competitor", he said. It was just never meant to be I guess.
Which he duly did, leaving Blake searching for answers.
"I believed in myself, that I could get the gold medal", Simbine told the BBC. "I couldn't recover from it".
"Nothing threw me off, it was just stumbling all the way through". Only. He is the fastest man in the world now still competing, but for 60 metres Australia's Trae Williams was quicker than him.
"I was stumbling all the way and I didn't recover from it", he said.
"It is an incredible feeling to come here and do what I could have done, and I've won the gold medal which is something I knew I could do".
Trinidad and Tobago's Michelle-Lee Ahye triumphed in the women's race, her first gold medal at a major global meet.
Trinidadian Michelle-Lee Ahye won semi-final two in 11.25, with Jamaican Gayon Evans following her home in 11.37. (Getty) Ahye led from start to finish to win in 11.14 seconds.
It was the first time in 20 years the 100m crown was not retained by a Caribbean nation. "Tomorrow I'll go out there and do the job", said Blake.
"I just stayed calm and did what I had to do".
Uganda's Stella Chessang won a closely contested women's 10,000m in 31:45.30, and world champion Tomas Walsh heaved 21.41m to win the men's shot put for New Zealand.In swimming, South Africa's Chad le Clos completed a hat-trick of butterfly titles when he won the 100m race, and Bronte Campbell earned a rare victory over her sister Cate in the 100m freestyle.
Chesang snapped a Kenyan stranglehold in the event which had dated back to 1998.