Amazon is only showing 20 live matches per season starting in 2019 on Prime Video, which is available through a standard Prime membership at £79 a year or standalone Prime Video membership at £7.99 a month in the United Kingdom, as well as weekly highlights of the matches throughout the season.
Sky will continue to show the most games after agreeing in February to pay 3.58 billion pounds for 128 matches a season for three years, including new Saturday evening fixtures.
The rights include one round of midweek games and one round on a bank holiday in December. Sky won four of the five "best deals", with BT taking the other.
The remaining rights to show another two rounds of midweek games were purchased by BT Sport for 90 million pounds ($121 million), giving it 52 live matches from 2019 to 2022.
It will offer the matches as part of its Amazon Prime Video subscription, it is said, with all members getting access to the live streams at no extra cost.
"Undoubtedly, Amazon is pulling together a strong and growing set of live TV rights to complement its vast content relationship", he said.
Premier League Executive Chairman Richard Scudamore said: "We welcome Amazon as an exciting new partner and we know Prime Video will provide an excellent service on which fans can consume the Premier League".
This year's Wimbledon tennis championships and US Open Tennis will also be available on demand on Amazon Prime in the United Kingdom, the company said.
He cautioned that "Amazon's current business model is unsustainable to support the acquisition of costly live sports rights in the long term" and predicted that Amazon will introduce a new sports channel bundle for Prime subscribers. Prime costs the equivalent of $106 per year in Britain.
Any change to the Premier League's rules needs a two-thirds majority - at least 14 of the 20 clubs - and the big six failed to achieve this at a league shareholders' meeting in October, prompting fears that the league's successful approach to selling its rights collectively was about to collapse, with some suggesting the big six could be tempted to form a European super league.
However, Amazon's foray into Premier League broadcasting should not be seen as a long-term threat to Sky and BT, Liberum media analyst Iain Whittaker insisted.
The Premier League is easing into doling out fixtures to streaming video.
The revenue from British rights is not distributed entirely on an equal basis with clubs given more according to league position and also the amount of times they feature on live broadcasts.